DIY for Mi-Hy
To promote sustainable agriculture, Mi-Hy is developing a do-it-yourself prototype.
We’re using our accessible circular hydroponic system to encourage and inspire the DIY community through a series of workshops and detailed tutorials.
You can find tutorials for MFCs and hydroponics here, as well as FAQs, workshop announcements and supporting materials.
Events and workshops:
MFC workshop at Open Hardware Summit, Berlin
Mi-Hy is joining this year's Open Hardware Summit with a microbial fuel cell building tutorial. Taking place in Technische Universität Berlin on the...
Open call: MudTronics microbial fuel cell championship
Mi-Hy is launching MudTronics, a collaborative competition at the intersection of biology, technology, and art/design. Participants explore how...
Online SPIKA Workshop at the Future Tech Week 2026, Jan 22
On January 22, an online workshop focusing on SPIKA will take place as part of Future Tech Week 2026. Running from January 19 to January 22, this...
Mi-Hy at Science Night and Maker Faire in Liberec and Mladá Boleslav, Sep 26-27
On September 26th and 27th, the Mi-Hy project traveled through the Czech Republic for two back-to-back events. First, on the 26th, it was featured...
How does Mi-Hy system work?

Mi-Hy (Microbial Hydroponics) is a circular system in which microbes treat wastewater and recycle it into electricity and fertilizer for plant growth. It consists of two main parts:
1. A microbial fuel cell (MFC) module with bacteria where the wastewater is added to;
2. A hydroponic setup in which plants grow in water treated with the MFC with recovered elements in it and light powered by the MFCs.
MFC tutorial and FAQs:
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What is MFC?

Microbial fuel cells (MFCs) use living microbes to convert chemical energy stored in organic matter into electrical energy. It consists of anode and cathode compartements with a semi-permiebale membrane between them:
In the anode compartment, microorganisms oxidize organic compounds under anaerobic conditions, releasing electrons and protons. The electrons are collected by the anode material and transported via an external circuit, while protons and/or positively charged ions migrate internally through the membrane. At the cathode, electrons and protons recombine with oxygen to form water, completing the redox cycle.
What are applications of MFCs?
While each MFC produces low power, individual units can be connected in series to increase voltage, in parallel to boost current and as a combination, allowing tailored configurations for different uses. A small array of about ten cells is enough to power low‑energy devices such as environmental sensors, microcontrollers, LoRa communication nodes, and LEDs for continuous, ultra‑low‑power operation.
Big stacks can be used for autonomous lighting in remote areas: like in this school in Uganda.
How much energy do MFCs produce?
Individual unit performance in labs reaches 0.5 V, however output varies depending on the dimensions and materials used. Usually MFCs are connected in several stacks of 20-24 units that can power devices or lights – see our partner’s collaboration with Glastonbury festival.
We have prepared an open-science version of the microbial fuel cells developed within Mi-Hy project. It uses accessible materials and doesn’t require special equipment.
Download microbial fuel cell step-by-step instruction:
Microbial fuel cell video tutorial:
Calculators for MFC building:
For 1 L solution: 1 L aqua dest + 10 g Tryptophan + 5 g Yeast Extract + 1.64 g Acetate
Surface area dimensioning · Cathode : Anode ratio
Cathode
Anode
Ratio
Cathode area: —
Anode area: —
Anode height: —
Effective ratio: 1 : —
Carbon Veil Impregnation
Dilute PTFE
Cathode Mix
PTFE / activated carbon calculation
Hydroponics tutorial and FAQs:
Your Title Goes Here
Your content goes here. Edit or remove this text inline or in the module Content settings. You can also style every aspect of this content in the module Design settings and even apply custom CSS to this text in the module Advanced settings.
What is hydroponics?
Hydroponics is a soil-free cultivation method that grows plants in a nutrient-rich, water-based solution, often using inert substrates (like coconut coir or perlite) for mechanical support.
What is in the wastewater treated by the MFC?
As wastewater is being digested by bacteria and protons flow through the membrane, catholyte accumulates in the catholyte chamber. It contains treated water and recycled organic compounds – mainly nitrogen, potassium and phosphate. Our tests have shown that MFCs efficiently filter out pathogens and microplastics.
Hydroponics video tutorial:
Further reading:
M.J. Salar-García, I. Ieropoulos. (2020). Optimisation of the internal structure of ceramic membranes for electricity production in urine-fed microbial fuel cells. Journal of Power Sources, 451.
Iwona Gajdaa, John Greenmana, Chris Melhuish, Ioannis Ieropoulos (2015). Simultaneous electricity generation and microbially-assisted electrosynthesis in ceramic MFCs. Bioelectrochemistry, 104, 58-64.
Dibyojyoty Nath, Ioannis Ieropoulos (2025). Electricity and fertiliser production using microbial fuel cell stacks for possible integration with hydroponics. Journal of Power Sources, 652
Ioannis A. Ieropoulos, Daniela Zertuche Moreno (2025). Microbial fuel cells powering robots and beyond; historical perspective and future directions. Bioelectrochemistry, 166
Keegan G. Cooke, Marcus O. Gay, Sage E. Radachowsky, Juan J. Guzman, and Michael A. Chiu (2010). BackyardNet™: Distributed Sensor Network Powered by Terrestrial Microbial Fuel Cell Technology. Proceedings: SPIE Defense, Security, and Sensing
Juan J. Guzman, Keegan G. Cookea, Marcus O. Gaya, Sage E. Radachowskya, Peter R. Girguisb, and Michael A. Chiua (2010). Benthic Microbial Fuel Cells: Long-Term Power Sources for Wireless Marine Sensor Networks. Proceedings: SPIE Defense, Security, and Sensing
T. Brooks, C. W. Keevil (1997). A simple artificial urine for the growth of urinary pathogens. Letters in AppliedMicrobiology, 24, 203–206
Building a Winogradsky Column: An Educator Guide with Activities in Astrobiology. D. Bodony, A. Chaussee, B. Samuelson
MudWatt Hackerboard Tutorial. MudWatt
How does Mi-Hy system work?

Mi-Hy (Microbial Hydroponics) is a circular system in which microbes treat wastewater and recycle it into electricity and fertilizer for plant growth. It consists of two main parts:
1. A microbial fuel cell (MFC) module with bacteria where the wastewater is added to;
2. A hydroponic setup in which plants grow in water treated with the MFC with recovered elements in it and light powered by the MFCs.
MFC tutorial:
Your Title Goes Here
Your content goes here. Edit or remove this text inline or in the module Content settings. You can also style every aspect of this content in the module Design settings and even apply custom CSS to this text in the module Advanced settings.
What is an MFC?

Microbial fuel cells (MFCs) use living microbes to convert chemical energy stored in organic matter into electrical energy. It consists of anode and cathode compartements with a semi-permiebale membrane between them:
In the anode compartment, microorganisms oxidize organic compounds under anaerobic conditions, releasing electrons and protons. The electrons are collected by the anode material and transported via an external circuit, while protons and/or positively charged ions migrate internally through the membrane. At the cathode, electrons and protons recombine with oxygen to form water, completing the redox cycle.
What are applications of MFCs?
While each MFC produces low power, individual units can be connected in series to increase voltage, in parallel to boost current and as a combination, allowing tailored configurations for different uses. A small array of about ten cells is enough to power low‑energy devices such as environmental sensors, microcontrollers, LoRa communication nodes, and LEDs for continuous, ultra‑low‑power operation.
Big stacks can be used for autonomous lighting in remote areas: like in this school in Uganda.
How much energy do MFCs produce?
Individual unit performance in labs reaches 0.5 V, however output varies depending on the dimensions and materials used. Usually MFCs are connected in several stacks of 20-24 units that can power devices or lights – see our partner’s collaboration with Glastonbury festival.
We have prepared an open-science version of the microbial fuel cells developed within Mi-Hy project. It uses accessible materials and doesn’t require special equipment.
Download microbial fuel cell step-by-step instruction:
Microbial fuel cell video tutorial:
Calculators for MFC building:
For 1 L solution: 1 L aqua dest + 10 g Tryptophan + 5 g Yeast Extract + 1.64 g Acetate
Surface area dimensioning · Cathode : Anode ratio
Cathode
Anode
Ratio
Cathode area: —
Anode area: —
Anode height: —
Effective ratio: 1 : —
Carbon Veil Impregnation
Dilute PTFE
Cathode Mix
PTFE / activated carbon calculation
Hydroponics tutorial:
Your Title Goes Here
Your content goes here. Edit or remove this text inline or in the module Content settings. You can also style every aspect of this content in the module Design settings and even apply custom CSS to this text in the module Advanced settings.
What is hydroponics?
Hydroponics is a soil-free cultivation method that grows plants in a nutrient-rich, water-based solution, often using inert substrates (like coconut coir or perlite) for mechanical support.
What is in the wastewater treated by the MFC?
As wastewater is being digested by bacteria and protons flow through the membrane, catholyte accumulates in the catholyte chamber. It contains treated water and recycled organic compounds – mainly nitrogen, potassium and phosphate. Our tests have shown that lab-built MFCs efficiently filter out pathogens and microplastics.
Hydroponics video tutorial:
Further reading:
M.J. Salar-García, I. Ieropoulos. (2020). Optimisation of the internal structure of ceramic membranes for electricity production in urine-fed microbial fuel cells. Journal of Power Sources, 451.
Iwona Gajdaa, John Greenmana, Chris Melhuish, Ioannis Ieropoulos (2015). Simultaneous electricity generation and microbially-assisted electrosynthesis in ceramic MFCs. Bioelectrochemistry, 104, 58-64.
Dibyojyoty Nath, Ioannis Ieropoulos (2025). Electricity and fertiliser production using microbial fuel cell stacks for possible integration with hydroponics. Journal of Power Sources, 652
Ioannis A. Ieropoulos, Daniela Zertuche Moreno (2025). Microbial fuel cells powering robots and beyond; historical perspective and future directions. Bioelectrochemistry, 166
Keegan G. Cooke, Marcus O. Gay, Sage E. Radachowsky, Juan J. Guzman, and Michael A. Chiu (2010). BackyardNet™: Distributed Sensor Network Powered by Terrestrial Microbial Fuel Cell Technology. Proceedings: SPIE Defense, Security, and Sensing
Juan J. Guzman, Keegan G. Cookea, Marcus O. Gaya, Sage E. Radachowskya, Peter R. Girguisb, and Michael A. Chiua (2010). Benthic Microbial Fuel Cells: Long-Term Power Sources for Wireless Marine Sensor Networks. Proceedings: SPIE Defense, Security, and Sensing
T. Brooks, C. W. Keevil (1997). A simple artificial urine for the growth of urinary pathogens. Letters in AppliedMicrobiology, 24, 203–206
Building a Winogradsky Column: An Educator Guide with Activities in Astrobiology. D. Bodony, A. Chaussee, B. Samuelson
MudWatt Hackerboard Tutorial. MudWatt
Events and workshops:
MFC workshop at Open Hardware Summit, Berlin
Mi-Hy is joining this year's Open Hardware Summit with a microbial fuel cell building tutorial. Taking place in Technische Universität Berlin on the...
Open call: MudTronics microbial fuel cell championship
Mi-Hy is launching MudTronics, a collaborative competition at the intersection of biology, technology, and art/design. Participants explore how...